Composite
Part:BBa_K2533000:Design
Designed by: Ziyang Xiao Group: iGEM18_HUST-China (2018-09-08)
RBS-ldhD-RBS-lldP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 780
Illegal BglII site found at 1325 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1461
Illegal AgeI site found at 1924
Illegal AgeI site found at 2362
Illegal AgeI site found at 2521
Illegal AgeI site found at 2533 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1035
Design Notes
LdhD is lactate dehydrogenase gene. LldP protein gene is used to transport the lactate out of the cell. We successfully construct genes ldhD-lldp using molecular biology experiment methods.
Source
From Lactobacillus bulgaricus.
References
[1]Li, C., Tao, F., Ni, J., Wang, Y., Yao, F., & Xu, P. (2015). Enhancing the light-driven production of d-lactate by engineering cyanobacterium using a combinational strategy. Scientific Reports, 5, 9777. http://doi.org/10.1038/srep09777